Aerial RGB image processing protocol for identifying potential maize genotypes in the vegetative stage

Authors

Keywords:

Plant breeding, Vegetation indices, Plant breeding. Vegetation indices. Zea mays L.

Abstract

Abstract: The aim was to evaluate an RGB image for identify grain yield potential in maize before physiological maturity in a semi-arid region. A randomized complete block design with two replications was employed, to access 50 maize genotypes. Two drone flights were conducted at different time points, specifically 27 and 46 days after planting (DAP), at flight heights of 40, 60, and 80 meters. A total of 29 vegetation indices were used in the analysis. Analysis of variance revealed genetic variability among the genotypes, enabling the selection of promising materials. By assessing the repeatability of vegetation indices, the optimal flight date was determined. Temporal BLUP (Best Linear Unbiased Prediction) allowed for the categorization of materials as high or low performers, considering the mean grain yield, and identified the most productive materials during the vegetative phenological stage of the crop. It is recommended, to conduct flights at 27 DAP at an height of 80 meters. The TGI (Triangular Greenness Index) and Green indices proved to be indicative of early predictions of material productivity. It is suggested to maintain the experimental area free from biotic and abiotic interferences and to conduct additional flights, thus optimizing aerial image phenotyping.

Downloads

Download data is not yet available.

Author Biographies

Barbara Nascimento Santos, Federal University of Sergipe (UFS)

Master's student in Agriculture and Biodiversity at the Federal University of Sergipe – UFS. Email: barbaranascimento2804@gmail.com. ORCID: https://orcid.org/0000-0002-1689-7399.

Nartênia Susane Costa Aragão, Federal University of Sergipe (UFS)

Student of Agricultural Engineering at the Federal University of Sergipe – UFS. Email: nartenia.aragao@gmail.com. ORCID: https://orcid.org/0000-0003-3409-3236.

Mário Sérgio Rodrigues Barreto, Federal University of Sergipe (UFS)

Student of Agricultural Engineering at the Federal University of Sergipe – UFS. Email: mzs.esc@gmail.com. ORCID: https://orcid.org/0000-0002-4037-4328.

Henrique Rocha Azevedo Santos, Federal University of Sergipe (UFS)

Student of Agricultural Engineering at the Federal University of Sergipe – UFS. Email: rique999@academico.ufs.br.

Jacilene Francisca Souza Santos, Federal University of Sergipe (UFS)

PhD student in Agriculture and Biodiversity at the Federal University of Sergipe – UFS. Email: jacilenesantos_14@hotmail.com.

José Jairo Florentino Cordeiro Junior, Federal University of Sergipe (UFS)

PhD in Agricultural Engineering. Professor at the Federal University of Sergipe – UFS. Email: jairofcordeiro@academico.ufs.br. ORCID: https://orcid.org/0000-0002-1138-8309.

Gustavo Hugo Ferreira de Oliveira, Federal University of Sergipe (UFS)

PhD in Agronomy. Professor at the Federal University of Sergipe – UFS. Email: gustavooliveira@academico.ufs.br. ORCID: https://orcid.org/0000-0002-3839-6261.

References

ALVARES, C. A. et al. Koppen’s climate classification map for Brazil. Meteorologische Zeitschrift. v. 22, n. 6, p. 711-728, 2013. Disponível em: http://dx.doi.org/10.1127/0941-2948/2013/0507. Acesso em: 22 jan. 2024.

ANDERSON, S. L. et al. Prediction of maize grain yield before maturity using improved temporal height estimates of unmanned aerial systems. The Plant Phenome Journal, v. 2, n. 1, p. 1-15, 2019. Disponível em: https://doi.org/10.2135/tppj2019.02.0004. Acesso em: 22 jan. 2023.

ASADZADEH, S.; SOUZA FILHO, C. R. Investigating the capability of WorldView-3 superspectral data for direct hydrocarbon detection. Remote Sensing of Environment, v. 173, p. 162-173, 2016. Disponível em: https://doi.org/10.1016/j.rse.2015.11.030. Acesso em: 22 jan. 2024.

BENDIG, J. et al. Combining UAV-based plant height from crop surface models, visible, and near infrared vegetation indices for biomass monitoring in barley. International Journal of Applied Earth Observation and Geoinformation, v. 39, p. 79-87, 2015. Disponível em: https://doi.org/10.1016/j.jag.2015.02.012. Acesso em: 22 jan. 2024.

BURGOS-ARTIZZU, X. P. et al. Real-time image processing for crop/weed discrimination in maize fields. Computers and Electronics in Agriculture, v. 75, n. 2, p. 337-346, 2011. Disponível em: https://doi.org/10.1016/j.compag.2010.12.011. Acesso em: 22 jan. 2024.

CECCATO, P. et al. Detecting vegetation leaf water content using reflectance in the optical domain. Remote sensing of environment, v. 77, n. 1, p. 22-33, 2001. Disponível em: https://doi.org/10.1016/S0034-4257(01)00191-2. Acesso em: 22 jan. 2024.

CONAB. Companhia Nacional de Abastecimento. Acompanhamento da Safra Brasileira de Grãos, Brasília, DF, v. 10, safra 2022/23, n. 8 oitavo levantamento, maio 2023. Disponível em: https://www.conab.gov.br/info-agro/safras/graos/boletim-da-safra-de-graos. Acesso em 15 mai. 2023.

FARIAS NETO, J. T.; YOKOMIZO, G.; BIANCHETTI, A. Coeficientes de repetibilidade genética de caracteres em pupunheira. Revista Brasileira de Fruticultura, v. 24, p. 731-733, 2002. Disponível em: https://doi.org/10.1590/S0100-2945200200030004. Acesso em: 22 jan. 2024.

FURUYA, D. E. G. et al. Prediction of insect-herbivory-damage and insect-type attack in maize plants using hyperspectral data. International Journal of Applied Earth Observation and Geoinformation, v. 105, p. 102608, 2021. Disponível em: https://doi.org/10.1016/j.jag.2021.102608. Acesso em: 22 jan. 2024.

GITELSON, A. A. et al. Novel algorithms for remote estimation of vegetation fraction. Remote sensing of Environment, v. 80, n. 1, p. 76-87, 2002. Disponível em: https://doi.org/10.1016/S0034-4257(01)00289-9. Acesso em: 22 jan. 2024.

GUIJARRO, M. et al. Automatic segmentation of relevant textures in agricultural images. Computers and Electronics in Agriculture, v. 75, n. 1, p. 75-83, 2011. Disponível em: https://doi.org/10.1016/j.compag.2010.09.013. Acesso em: 22 jan. 2024.

GURGEL, F. L.; FERREIRA, D. F.; SOARES, A. C. S. O coeficiente de variação como critério de avaliação em experimentos de milho e feijão. Belém, PA: Embrapa Amazônia Oriental-Boletim de Pesquisa e Desenvolvimento, p. 80, 2013. Disponível em: https://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/955896. Acesso: em 15 mai. 2023.

HAGUE, T.; TILLETT, N. D.; WHEELER, H. Automated crop and weed monitoring in widely spaced cereals. Precision Agriculture, v. 7, p. 21-32, 2006. Disponível em: https://doi.org/10.1007/s11119-005-6787-1. Acesso em: 22 jan. 2024.

HERZIG, P.; et al. Evaluation of RGB and Multispectral Unmanned Aerial Vehicle (UAV) Imagery for High-Throughput Phenotyping and Yield Prediction in Barley Breeding. Remote Sensing, v. 13, n. 14, p. 2670, 7 jul. 2021. Disponível em: https://doi.org/10.3390/rs13142670. Acesso em: 22 jan. 2024.

HUNT, E. R. et al. Evaluation of digital photography from model aircraft for remote sensing of crop biomass and nitrogen status. Precision Agriculture, v. 6, p. 359-378, 2005. Disponível em: https://doi.org/10.1007/s11119-005-2324-5. Acesso em: 22 jan. 2024.

HUNT JR, E. R. et al. Remote sensing leaf chlorophyll content using a visible band index. Agronomy journal, v. 103, n. 4, p. 1090-1099, 2011. Disponível em: https://doi.org/10.2134/agronj2010.0395. Acesso em: 22 jan. 2024.

HU, P. et al. Phenomic selection and prediction of maize grain yield from near‐infrared reflectance spectroscopy of kernels. The Plant Phenome Journal, v. 3, n. 1, p. e20002, 2020. Disponível em: https://doi.org/10.1002/ppj2.20002. Acesso em: 22 jan. 2024.

LANE, H. M. et al. Phenomic selection and prediction of maize grain yield from near‐infrared reflectance spectroscopy of kernels. The Plant Phenome Journal, v. 3, n. 1, p. e20002, 2020. Disponível em: https://doi.org/10.1002/ppj2.20002. Acesso em: 22 jan. 2024.

LIU, J. G.; MOORE, J. McM. Hue image RGB colour composition. A simple technique to suppress shadow and enhance spectral signature. International Journal of Remote Sensing, v. 11, n. 8, p. 1521-1530, 1990. Disponível em: https://doi.org/10.1080/01431169008955110. Acesso em: 22 jan. 2024.

LOUHAICHI, M; BORMAN, M. M.; JOHNSON, D. E. Spatially located platform and aerial photography for documentation of grazing impacts on wheat. Geocarto International, v. 16, n. 1, p. 65-70, 2001. Disponível em: https://doi.org/10.1080/10106040108542184. Acesso em: 22 jan. 2024.

KATAOKA, T. et al. Crop growth estimation system using machine vision. In: Proceedings 2003 IEEE/ASME international conference on advanced intelligent mechatronics (AIM 2003). IEEE, v. 2, 2003. p. b1079-b1083. Disponível em: https://doi.org/10.1109/AIM.2003.1225492. Acesso em: 22 jan. 2024.

KAWASHIMA, S.; NAKATANI, M. An algorithm for estimating chlorophyll content in leaves using a video camera. Annals of Botany, v. 81, n. 1, p. 49-54, 1998. Disponível em: https://doi.org/10.1006/anbo.1997.0544. Acesso em: 22 jan. 2024.

MAES, W. H.; STEPPE, K. Perspectives for remote sensing with unmanned aerial vehicles in precision agriculture. Trends in plant science, v. 24, n. 2, p. 152-164, 2019. Disponível em: https://doi.org/10.1016/j.tplants.2018.11.007. Acesso em: 22 jan. 2024.

MEYER, G. E. et al. Intensified fuzzy clusters for classifying plant, soil, and residue regions of interest from color images. Computers and electronics in agriculture, v. 42, n. 3, p. 161-180, 2004. Disponível em: https://doi.org/10.1016/j.compag.2003.08.002. Acesso em: 22 jan. 2024.

MEYER, G. E.; NETO, J. C. Verification of color vegetation indices for automated crop imaging applications. Computers and electronics in agriculture, v. 63, n. 2, p. 282-293, 2008. Disponível em: https://doi.org/10.1016/j.compag.2008.03.009. Acesso em: 22 jan. 2024.

PEREZ, A. J. et al. Colour and shape analysis techniques for weed detection in cereal fields. Computers and electronics in agriculture, v. 25, n. 3, p. 197-212, 2000. Disponível em: https://doi.org/10.1016/S0168-1699(99)00068-X. Acesso em: 22 jan. 2024.

QIU, R. et al. Detection of the 3D temperature characteristics of maize under water stress using thermal and RGB-D cameras. Computers and Electronics in Agriculture, v. 191, p. 106551, 2021. Disponível em: https://doi.org/10.1016/j.compag.2021.106551. Acesso em: 22 jan. 2024.

SANTANA, D. C. et al. UAV-based multispectral sensor to measure variations in corn as a function of nitrogen topdressing. Remote Sensing Applications: Society and Environment, v. 23, p. 100534, 2021. Disponível em:https://doi.org/10.1016/j.rsase.2021.100534. Acesso em: 22 jan. 2024.

SANTOS, E. F. N.; SOUSA, I. F. D.; LEITE, I. V. Regiões Homogêneas em Sergipe Agrupadas Através dos índices Climáticos. Revista Brasileira de Meteorologia, v. 37, n. 4, p. 477–489, out. 2022. Disponível em: http://dx.doi.org/10.1590/0102-77863740053. Acesso em: 22 jan. 2024.

SILVA, A. F.; REGITANO NETO, A.; NETO, A. R. As principais culturas anuais e bianuais na agricultura familiar. Agricultura Familiar, p. 45, 2019. Acesso em: 22 jan. 2024.

TETILA, E. C. et al. Detection and classification of soybean pests using deep learning with UAV images. Computers and Electronics in Agriculture, v. 179, p. 105836, 2020. Disponível em: https://doi.org/10.1016/j.compag.2020.105836. Acesso em: 22 jan. 2024.

TUCKER, C. J. Red and photographic infrared linear combinations for monitoring vegetation. Remote sensing of Environment, v. 8, n. 2, p. 127-150, 1979. Disponível em: https://doi.org/10.1016/0034-4257(79)90013-0. Acesso em: 22 jan. 2024.

WOEBBECKE, D. M. et al. Color indices for weed identification under various soil, residue, and lighting conditions. Transactions of the ASAE, v. 38, n. 1, p. 259-269, 1995. Disponível em: https://doi.org/10.13031/2013.27838. Acesso em: 22 jan. 2024.

WOEBBECKE, D. M. et al. A. Shape features for identifying young weeds using image analysis. Transactions of the ASAE, v. 38, n. 1, p. 271-281, 1995. Disponível em: https://doi.org/10.13031/2013.27839. Acesso em: 22 jan. 2024.

WILBER, A. L.; CZARNECKI, J. M. P.; MCCURDY, J. D. An ArcGIS Pro workflow to extract vegetation indices from aerial imagery of small plot turfgrass research. Crop Science, 62, 503–511, 2022. Disponível em: https://doi.org/10.1002/csc2.20669. Acesso em: 22 jan. 2024.

ZARCO-TEJADA, P. J. et al. Assessing vineyard condition with hyperspectral indices: Leaf and canopy reflectance simulation in a row-structured discontinuous canopy. Remote Sensing of Environment, v. 99, n. 3, p. 271-287, 2005. Disponível em: https://doi.org/10.1016/j.rse.2005.09.002. Acesso em: 22 jan. 2024.

Published

2012-12-31

How to Cite

NASCIMENTO SANTOS, Barbara; COSTA ARAGÃO, Nartênia Susane; RODRIGUES BARRETO, Mário Sérgio; ROCHA AZEVEDO SANTOS, Henrique; SOUZA SANTOS, Jacilene Francisca; FLORENTINO CORDEIRO JUNIOR, José Jairo; FERREIRA DE OLIVEIRA, Gustavo Hugo. Aerial RGB image processing protocol for identifying potential maize genotypes in the vegetative stage. Electronic Journal Digital Skills for Family Farming, Tupã, São Paulo, Brasil, v. 9, n. 2, p. 149–173, 2012. Disponível em: https://owl.tupa.unesp.br/recodaf/index.php/recodaf/article/view/178. Acesso em: 21 may. 2024.